A Clostridium acetobutylicum regulator gene (regA) affecting amylase production in Bacillus subtilis
نویسندگان
چکیده
منابع مشابه
A Clostridium acetobutylicum regulator gene (regA) affecting amylase production in Bacillus subtilis.
Plasmid pMET7C containing a 6.05 kb DNA insert from Clostridium acetobutylicum P262 made Escherichia coli F19 cells sensitive to metronidazole. The nucleotide sequence of the C. acetobutylicum DNA controlling metronidazole sensitivity in E. coli F19 revealed an ORF of 972 bp which encoded a protein of 324 amino acids with a calculated Mr of 35,000. The amino acid sequence encoded by the ORF con...
متن کاملThe amylase of Clostridium acetobutylicum.
The purification and concentration of enzymes by adsorption on charcoal, tannin, aluminum hydroxide, ion exchange resins, and so forth, has been an established practice in the laboratory. Some years before this work was done in 1949, alkali-cooked lignin was used as an adsorbent for mold and malt amylase (Wallerstein et al., 1945). In this paper is described our study of the adsorption of the a...
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This work concerns the degradation of starch to glucose by enzymes of Clostridium acetobutylicum, a stage in the butanol-acetone fermentation which has so far been little investigated. Previous workers have mainly used growing cultures of Cl. acetobutylicum, though Robinson (1922) showed that starch (and other carbohydrates) were fermented by toluene-treated cell suspensions. In the present wor...
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A segregated model was proposed to investigate the inherent relationships between growth, substrate consumption, cell differentiation and product formation in a Bacillus subtilis fermentation producing heterologous amylase in a 22-l bioreactor. The segregated model includes three distinguishable cell states and the transition from vegetative phase to sporangium and finally to mature spore. An a...
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We produced a-amylase from banana peel by Bacillus subtilis through solid state fermentation (SSF). Addition of yeast extract, corn steep liquor, sodium dodesyl sulphate (SDS) and Tween-80 enhanced the a-amylase production under preoptimized fermentation conditions. Maximum enzyme yield (10.25 U/mL) was observed at 35°C after 24 hours of SSF in growth medium containing 0.1% yeast extract, 1% co...
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ژورنال
عنوان ژورنال: Microbiology
سال: 1995
ISSN: 1350-0872,1465-2080
DOI: 10.1099/13500872-141-4-989